Injection of tissue plasminogen activator into the optic nerve in an animal model of retinal vein occlusion

姜彩辉   张卯年   瓶井   资弘

解放军总医院眼科   日本大阪大学医学院眼科   100853

AIM：To investigate the feasibility and efficacy of tissue plasminogen activator(tPA)injection into the optic nerve as a treatment for retinal vein occlusion in rabbits.
METHODS：Rose Bengal-mediated laser-induced RVO were produced in rabbit eyes.FA was performed on each eye 3 days before laser irradiation and 30 minutes after laser irradiation.The treatment group(n=20 veins)received intra-optic nerve injection of tPA(12.5 μg in 0.05ml BSS)and the controls(n=24 veins)received 0.05ml BSS.FA was repeated to determine the recanalization of the vessel at 3 and 7 days after treatment，followed by histological examination.

RESULTS：Rose Bengal-mediated laser-induced RVO were successfully developed and confirmed by fluorescein angiography(FA).The incidence of the recanalization of the vessels in treatment animals was 70.0%，while 16.7%in the control animals(P=0.001).
CONCLUSION：Intra-optic nerve tPA injection increased the incidence of recanalization of the occluded vessels.Although further studies are needed，our data suggested that injection of tPA into the optic nerve may have a potential for the treatment of CRVO.