【摘要】  目的：探讨增殖性糖尿病视网膜病变(PDR)中组织纤溶酶原激活物(t-PA)及纤溶酶原激活物抑制剂(PAI)的表达以及与血管内皮生长因子(VEGF)表达的相关性。方法：于玻璃体手术中采集PDR 35眼玻璃体，同时采集因黄斑裂孔行玻璃体手术20眼玻璃体作为对照组，采用酶联免疫吸附法检测t-PA及PAI的表达浓度，并与VEGF的表达进行相关性分析。结果：PDR眼玻璃体中VEGF、t-PA及PAI的表达浓度均显著高于对照眼玻璃体(P <0.001)。 t-PA及PAI的表达与VEGF的表达经统计学分析，均存在显著相关性 (P <0.001)。结论：在PDR眼内视网膜新生血管的发生过程中不仅有VEGF，可能同时有多种生物活性物质的参与。

【关键词】  糖尿病视网膜病变；VEGF；组织纤溶酶原激活物；纤溶酶原激活物抑制剂；血管生成

    The correlation study of the expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) with VEGF expression in proliferative diabetic retinopathy (PDR)

    Hai Lu, Feng Zhang

    Department of Ophthalmology, Beijing Tongren Hospital, Capital University of Medical Science, Beijing 100730, China

     AbstractAIM: To evaluate the correlation between the expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) with vascular endothelial growth factor (VEGF) expression in proliferative diabetic retinopathy (PDR).  METHODS: Vitreous samples were taken from 35 eyes with PDR and analyzed with enzyme linked immunosorbent assay (ELISA) for the expression of VEGF, t-PA and PAI. Control samples were from 20 eyes with idiopathic macular hole and analyzed in the same way. The correlation between VEGF expression and the expression of t-PA and PAI in proliferative diabetic retinopathy were studied.ESULTS: VEGF, t-PA and PAI were significantly expressed as compared with those in control subjects (P<0.001). The expression of t-PA and PAI were highly correlated with that of VEGF in proliferative diabetic retinopathy (P<0.001). CONCLUSION: It is suggested that a number of bioactive substances may be involved in the pathogenesis of angiogenesis in PDR. The expression of t-PA may be upregulated by the activation of VEGF, which may further facilitate the process of angiogenesis in PDR. Meanwhile, the correlated expression of PAI may suggest the presence of an endogenous angiogenesis mechanism accompanying the activities of VEGF.

    · KEYWORDS: diabetic retinopathy; vascular endothelial growth factor; tissue-plasminogen activator (t-PA); plasminogen activator inhibitor (PAI); angiogenesis

    卢海,张风.增殖性糖尿病视网膜病变眼内组织纤溶酶原激活物及其抑制物的表达与VEGF表达的相关性研究.

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