在本试验中,曲伏前列腺素作用后,设定对照组mRNA相对表达量为1作标准，6，12，24h组NF-кB p65mRNA相对表达量为0.492±0.100，0.515±0.173，0.784±0.030；免疫荧光半定量分析显示，对照组，6，12，24h组NF-кB p65荧光强度为176.65±4.34，119.90±12.54， 155.81±9.15，146.71±8.23，表现为睫状肌细胞内NF-кB表达在各时间组内明显降低；IкB初期改变不明显,24h后表达升高，6，12，24h组IkBɑ mRNA相对表达量为0.929±0.055，0.915±0.039，1.354±0.105，免疫荧光半定量分析显示对照组，6，12，24h组IкBɑ的荧光强度为107.62±7.26，99.16±8.67，94.82±5.33，129.67±8.08；并且对照组，6，12，24h组的磷酸化p65与p65的A比值均数分别为0.930±0.0235，0.870±0.086，0.748±0.012， 0.685±0.030，呈现磷酸化NF-кB p65含量随药物作用时间逐渐降低趋势，说明NF-кB p65的核易位受抑制。提示NF-кB在葡萄膜巩膜房水流出通道中可能起负调控作用。这可能与cAMP或cGMP抑制作用或细胞外基质的降解对其产生作用等多种调控机制有关。由于NF-кB广泛参与多种细胞因子和基因的转录调控，所以，NF-кB及其抑制因子IкB系统在葡萄膜巩膜通道中的上游及下游调控机制需进一步实验研究证实。

    综上所述， NF-кB在曲伏前列腺素药物作用于人睫状肌细胞后，表现为表达下降，核易位受抑制，提示NF-кB在葡萄膜巩膜房水流出通道中起负调控作用。能否应用NF-кB抑制物增加葡萄膜巩膜房水流出，将为青光眼的药物治疗开辟新的途径。

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