【摘要】  　　目的：观察不同浓度碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）引起体外培养的人晶状体上皮细胞迁移和黏着斑激酶（focal adhesion kinase，FAK）mRNA水平的动态变化。方法：体外培养的第3代人晶状体上皮细胞，建立细胞划痕损伤模型，经0，5，10，15μg/L bFGF作用8 ~16h后，电脑图像法测定细胞迁移距离，RT-PCR法检测0，1，4，8， 16h FAK mRNA水平，免疫荧光法检测整合素β1和增殖细胞核抗原表达阳性率的变化。结果：与对照组比，5μg/L组细胞迁移距离和FAK mRNA表达显著增加（P <0.05）；10μg/L组细胞整合素β1表达阳性率显著增加（P <0.05）；15μg/L细胞迁移距离和FAK mRNA显著降低（P <0.05）。结论：bFGF引起人晶状体上皮细胞迁移距离和FAK mRNA表达的变化。　

【关键词】  碱性成纤维细胞生长因子　上皮细胞　晶状体　黏着斑激酶　迁移

　　Effect of bFGF on expression focal adhesion kinase in human lens epithelial cells

    Xin-Ling Wang1, Tao Yu2, Guo-Gang Zhang3, Qi-Chang Yan1, Jin-Song Zhang1

    Foundation items: The 33th Postdoctoral Science Foundation of State Department, China (No. 2003033282); Scientific Research Project for Institute of Higher Education of Education Bureau, Liaoning Province, China (No. 2004C041); S&T Research Project for Institution of Higher Education Bureau, Liaoning Province, China (No. 05L576); Natureal Science Project for Postdoctor Launch, liaoning Province, China (No. 20062091)

    1Department of Ophthalmology, the Affiliated Fourth Hospital, China Medical University, Shenyang 110005. Liaoning Province, China;2 Department of Radiology, Liaoning Cancer Hospital, Shenyang 110042, Liaoning Province, China;3School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University, Shenyang 110016, Liaoning Province, China

    Abstract AIM: To observe the effects of different concentration basic fibroblast growth factor (bFGF) on human lens epithelial cells migration and the dynamic changes in focal adhesion kinase (FAK) mRNA level.METHODS: The scratch wound model of the third passage human lens epithelial cells was established and treated with bFGF of different concentrations (0, 5, 10 and 15μg/L) for 8-16 hour. The images of cell migration were quantitatively measured using a computer-assisted videomicroscopic system. The mRNA level of FAK was measured with reverse transcriptase-polymerase chain reaction (RT-PCR) assay at 0, 1, 4, 8 and 16 hour. The positive expression of integrin β1 and proliferating cell nuclear antigen (PCNA) were studied by immunofluorescent staining.RESULTS: The cell migration and FAK mRNA level were significantly stimulated when treated with bFGF at the concentrations of 5μg/L compared with the control group (P <0.05). The integrin β1 positive expression rate was significantly increased when treated with 10μg/L bFGF (P <0.05). The cell migration and FAK mRNA level was significantly inhibited when treated with 15μg/L bFGF (P <0.05).  CONCLUSION: The cell migration distance and FAK mRNA expression varies when the cells are treated with different concentration of bFGF. FAK plays an important role in human lens epithelial cells migration.

    · KEYWORDS: basic fibroblast growth factor; epithelial cell; lens; focal adhesion kinase; migration

　　0引言

    黏着斑激酶(focal adhesion kinase，FAK)是细胞黏着依赖的信号转导(adhesion-dependent signal transduction )中的酪氨酸蛋白激酶，属于非受体蛋白酪氨酸激酶，在大多组织细胞中皆有表达，参与调节细胞发育、生长、存活、凋亡、黏附、骨架重组等过程，是生长因子受体信号转导通路中的重要信号分子[1,2]。碱性成纤维细胞生长因子(basic fibroblast growth factor， bFGF) 是强效促细胞增殖因子，对细胞的黏附、迁移也有作用 [3,4]。我们探讨不同浓度bFGF引起体外培养的人晶状体上皮细胞损伤后迁移距离与FAK的动态变化，以揭示其对晶状体上皮细胞迁移与增殖的影响。

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