3  结语

　　基因芯片技术发展至今已经取得了巨大的成功，且仍然存在相当大的发展潜力。 基因芯片技术打破了以往“一种疾病一个基因” 的研究模式，弥补以往对复杂生命系统的研究总是采用单因素或多因素的分析方法的缺陷，能够充分利用所采集到的大量信息，整体宏观地研究生物体基因的表达及功能。该技术在眼科研究中用基因整体表达分析替代传统的单一基因，将有助于对复杂疾病如早期糖尿病视网膜病变的发病机制的理解。基因芯片在眼科的基础研究将更加围绕临床导向的疾病过程分析，成为眼科疾病重要的诊断工具和寻找药靶的依据。 相信随着该技术的快速发展及广阔应用，必然对眼科学产生更为深远的影响，有利于揭示眼科疾病发生、发展、治疗和预后等各方面的细胞途径以及遗传和分子机制。

　　【参考文献】

　　1  Edwin S,Kalim M,Mikhail S.Molecular interactions on microarrays.Nature Genetics Supplement,1999,21:5-9.

　　2  Cheng J,Fortina P,Surrey S,et al. Microchip based devices for molecular diagnosis of genetic disease. Mol Diagn,1996,1:183-200.

　　3  Kapp U.Interleukin 13 is secreted by and stimulates the growth of Hodgkin and Reed-Sternberg cells. J Exp Med,1999,189(12):1939-1946.

　　4  Hacia JG ． Resequencing and mutational analysis using oligonucleotide microarrays. Nat Genet，1999,21(1 Suppl):42-47.

　　5  Schena M,Shalon D,Davis RW,et al.Quantitative monitoring of gene expression patterns with acomplementary DNA microarray. Science,1995,270:467-470.

　　6  Guschin D,Yershov G, Zaslavsky A,et al. Manual manufacturing of oligonucleotide,DNA, and protein microchips.Anal Biochem,1997,250:203-211.

　　7  Borman S.DNA chip biochip construction and use in human genome mapping, mutation detection, disease diagnosis, gene expression analysis.Chem Eng News,1996,74:42-43.

　　8  Lueking A,Horn M,Eickhoff H,et al.Protein microarrys for gene expression and antibody screening. Anal Biochem,1999,270:103-111.

　　9  Lemieux B,Aharoni A,Schena M.Over view of DNA chip technology. Molec Breeding,1998,4:277-283.

　　10  Pease AC,Solas D,Sullivan EJ,et al.Light generated oligonucleotide arrays for rapid DNA sequence analysis. Proc Natl Acad Sci USA,1994,91:5022-5026.

　　11  Yershov G,Barsky V,Belgovskiy A,et al. DNA analysis and diagnostics on oligonucleotide microchips. Proc Natl Acad Sci USA,1996,93:4913-4918.

　　12  Livache T,Bazin H,Mathis G.Conducting polymers on microelectronic devices as tools for biological analyses. Clin Chim Acta,1998,278:171-176.

　　13  Livache T,Fouque B,Roget A,et al.Polypyrrole DNA chip on a silicon device:example of hepatitis virus genotyping.Anal Bio chem,1998,255:188-194.

　　14  Ferguson JA,Boles TC, Adams CP,et al. A fiber optic DNA biosensor microarray for the analysis of gene expression.Nat Biote chnol,1996,14:1681-1684.

　　15  Wen WH, Bernstein L, Lescallett J, et al. Comparison of TP53 mutations identified by oligonucleotide microarray and conventional DNA sequence analysis. Cancer Res,2000,60(10):2716-2722.

　　l6  Anthony RM.Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. J Clin Microbiol,2000,38(2):781-788.

　　17  Troesch A, Nguyen H, Miyada CG,et al. Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays. J Clin Microbiol,1999,37(1):49-55.

　　18  Farjo R, Yu J, Thman MI, et a1 ． Mouse eye gene microarrays for investigating ocular development and disease ． Vision Res, 2002, 42 ： 463-470．

　　19  Chowers I.Identification of novel genes preferentially expressed in the retina using a custom human retina cDNA microarray. Invest Ophthalmol Vis Sci,2003，44(9):3732-3741.

　　20  Li A, Zhu X, Brown B,et al. Gene expression networks underlying retinoic acid-induced differentiation of human retinoblastoma cells. Invest Ophthalmol Vis Sci,2003,44(3):996-1007.

　　21  Lenzner S,Prietz S,Feil S, et al.Global gene expression analysis in a mouse model for Norrie disease: late involvement of photoreceptor cells. Invest Ophthalmol Vis Sci, 2002,43(9):2825-2833.

　　22  Yoshida S, Yashar BM, Hiriyanna S,et al. Microarray analysis of gene expression in the aging human retina. Invest Ophthalmol Vis Sci,2002,43(8):2554-2560.

　　23  Singh S, Zheng JJ, Peiper SC,et al. Gene expression profile of ARPE-19 during repair of the monolayer. Graefes Arch Clin Exp Ophthalmol,2001,239(12):946-951.

　　24  Wistow G, Bernstein SL, Wyatt MK,et al. Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants. Mol Vis,2002,15(8):205-220.

　　25  Buraczynska M, Mears AJ, Zareparsi S,et al.Gene expression profile of native human retinal pigment epithelium. Invest Ophthalmol Vis Sci,2002,43(3):603-607.

　　26  Joussen AM, Huang S, Poulaki V, et al.In vivo retinal gene expression in early diabetes. Invest Ophthalmol Vis Sci,2001,42(12):3047-3057.

上一页  [1] [2]