白内障 3337 PO0067
Effect of transforming growth factor-β on the expression of connective tissue growth factor in bovine lens epithelial cells
CAO Yang, CHEN Bo, HU Yi Zhen
From the Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Technology and Science, Wuhan 430022, Hubei Province, China
[Abstract] Objective: To observe the effects of transforming growth factor-β(TGF-β)on the expression of connective tissue growth factor(CTGF) in bovine lens epithelial cells(BLECs).
Methods: Cultured 2-3 passage BLECs were divided into control group and experimental group with 0×103ng•L-1(control)、0.32×103ng•L-1、1.00×103ng•L-1、3.20×103ng•L-1 TGF-βfor 24h, the mRNA and protein of CTGF in BLEC were examined respectively by semiquantitative RT-PCR and Western blotting.
Results: In comparison with control group, the different concentration of TGF-βcould promote the expression of CTGF at mRNA and protein level in BLECs. The result of RT-PCR indicate that there was no significantly difference between the 0.32×103ng•L-1 group and control group(P>0.05), but there were significantly difference between the 1.00×103ng•L-1、3.20×103ng•L-1 group and control group(P<0.05). The result of Western blotting was conformed with the RT-PCR.
Conclusion: the data suggest that CTGF play an important role in lens epithelial cells migration, fibrosis and deposition of ECM in posterior capsule opacification.
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