基础             3336                                      PO0169

　　The expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells

　　CHEN Bo, HU Yi Zhen, CAO Yang

　　From the Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Technology and Science, Wuhan 430022, Hubei Province, China

　　Objective: To study the expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells(BTMC).

　　Methods: Reanimating the Pegfp-C1 strain, Pegfp-C1 was extracted and identified. The Pegfp-C1 was embedded in lipofectamine and transfected into BTMC. The fluorescence intensity of BTMC after transfected with EGFP gene was visualized by fluorescence microscope.

　　Results: Pegfp-C1 could be availably transfected into BTMC. After being transfected, BTMC with the EGFP gene displayed an effective EGFP expression.

　　Conclusions: An EGFP expressing plasmid was used to transfected BTMC. Transfected cells show a stable EGFP expression in vitro. This labeling method will be profited for studying BTMC through genic level.

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