综上所述,结合国内情况,泌尿生殖道Ct感染的实验诊断中,经典的细胞培养方法虽然特异,但耗时长、费用大,且不够敏感,不适于临床开展;免疫学方法简便快捷,在基层医院具有实用性;在具备分子生物学实验条件的医院,由于PCR方法高度敏感特异,可检测各发病率人群,只要严格规范操作,避免假阴性及假阳性结果的出现,可望在Ct感染诊断中常规使用。总之,Ct感染的诊断目前已发展到分子生物学水平,随着科学技术的不断进步,下个世纪有可能利用生物芯片等更新的技术进行检测。我们应密切注视检测技术的发展,以便准确、快速地对Ct感染作出诊断。
参考文献
1. 李凤鸣,主编.眼科全书.北京: 人民卫生出版社,1996.1305-1311.
2 黄策,端青.常见性传播疾病的实验检查及其进展.中国实用妇科与产科杂志,1998,14:86-88.
3 Mahony JB, Luinstra KE, Sellors JW, et al. Confirmatory polymerase chain reaction testing for Chlamydia trachomatis in first-void urine from asymptomatic and symptomatic men. J Clin Microbiol, 1992,30:2241-2245.
4 Pasternack R, Vuorinen P, Kuukankorpi A, and et al. Detection of Chlamydia trachomatis infections in women by Amplicor PCR: comparison of diagnostic performance with urine and cervical specimens. J Clin Microbiol, 1996,34:995-998.
5 姚兵,杨岳琴,朱启锭.套式聚合酶链反应结合直接荧光法检测女性生殖道沙眼衣原体.中华医学检验杂志,1998,21:338-341.
6 俞树荣,主编.微生物学和微生物学检验.第2版.北京:人民卫生出版社,1997.340-349.
7 Hallsworth PG, Hefford C, Waddell RG, et al. Comparison of antigen detection, polymerase chain reaction and culture for detection of Chlamydia trachomatis in genital infection. Pathology, 1995,27:168-171.
8 罗春丽,蔡晓钟,陈宏础,等.荧光单克隆抗体检测泌尿生殖道沙眼衣原体.上海医学检验杂志,1995,10:92-92.
9 LeBar WD. Keeping up with new technology: new approaches to diagnosis of Chlamydia infection. Clin Chem, 1996,42:809-812.
10 Buck GE. The polymerase chain reaction: a revolutionary new procedure for the laboratory diagnosis of infectious disease. J Ky Med Assoc,&nbs p;1996,94:148-152.
11 Schachter J. DFA,EIA,PCR,LCR and other technologies: what tests should be used for diagnosis of chlamydia infections? Immunol Invest, 1997,26:157-161.
12 Roosendaal R, Walboomers JM, Veltman OR, et al. Comparison of different primer sets for detection of Chlamydia trachomatis by the polymerase chain reaction. J Med Microbiol, 1993,38:426-433.
13 Palmer HM, Gilroy CB, Thomas BJ, et al. Detection of Chlamydia trachomatis by the polymerase chain reaction in swabs and urine from men with non-gonococcal urethritis. J Clin Pathol, 1991,44:321-325.
14 Lan J, Walboomers JM, Roosendaal R, et al. Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol, 1993,31:1060-1065.
15 Verkooyen RP, Luijendijk A, Huisman WM, et al. Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J Clin Microbiol, 1996,34:3072-3074.
16 Puolakkainen M, Hiltunen-Back E, Reunala T, et al. Comparison of performances of two commercially available tests, a PCR assay and a ligase chain reaction test, in detection of urogenital Chlamydia trachomatis infection. J Clin Microbiol, 1998,36:1489-1493.
17 Crotchfelt KA, Pare B, Gaydos C, et al. Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia trachomatis Assay (AMP CT) in urine specimens from men and women and endocervical specimens from women. J Clin Microbiol, 1998,36:391-394.
18 Stary A. Chlamydia screening: which sample for which technique? Genitourin Med, 1997,73:99-102.
19 Polaneczky M, Quigley C, Pollock L, et al. Use of self-collected vaginal specimens for detection of Chlamydia trachomatis infection. Obstet Gynecol, 1998,91:375-378.
20 Crotchfelt KA, Welsh LE, DeBonville D, et al. Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay. J Clin Microbiol, 1997,35:1536-1540.
21 颜进,彭红梅,刘剑雄.热启动双重聚合酶链反应检测淋病奈瑟菌和沙眼衣原体.临床检验杂志,1998,16:17-19.
22 郑占才,王家璧,王宏伟,等.复合PCR同时检测淋球菌沙眼衣原体和解脲支原体.中国皮肤性病学杂志,1998,12:328-330. 上一页 [1] [2] |