【摘要】 目的 探讨利用低分子肝素对内皮抑素进行结构修饰，并评价其对角膜新生血管(corneal neovascularization，CNV)的抑制作用。方法 采用化学方法用低分子肝素修饰内皮抑素，碱烧伤法建立兔角膜新生血管模型，分别在球结膜下注射低分子肝素化内皮抑素（Ⅰ组）、内皮抑素（Ⅱ组）和生理盐水（对照组），观察新生血管的生长情况。结果 成功的利用低分子肝素对内皮抑素进行了结构修饰，动物实验表明Ⅰ组新生血管的长度、面积、数量、VEGF的表达均明显低于其它两组，差异有统计学意义（P<0.05）。结论 低分子肝素化内皮抑素可有效抑制角膜新生血管的生长，效果优于内皮抑素。
【关键词】 低分子肝素 内皮抑素 修饰 角膜 新生血管 兔
its angiogenesis effects on rabbit corneas
ZHU Wei1, TAO Xiangchen2, LI Xin2, YUAN Zhongfang2, MU Guoying2
（1. School of Medicine, Shandong University, Jinan 250012， Shandong, China;
2. Department of Ophthalmology, Jinan Central Hospital, School of Clinical Medicine, Shandong University,
Jinan 250013, Shandong, China）
Abstract: Objective To investigate the modification of endostatin (ES) with low molecular weight heparin (LMEH) and its inhibitory effects on corneal neovascularization（CNV）. Methods ES was modified with LMWH by a chemical method. The rabbit CNV model was made with alkali burning. LMWHES(group 1)， ES (group 2) and normal saline(control group) were subconjunctivally injected. Results The blood vessel was shorter and smaller in group 1 than in the other two groups(P<0.05) and the expression of VEGF in group 1 was also significantly lower than that of the other two groups(P<0.05). Conclusions LMWHES can effectively inhibit the growth of CNV and is superior to ES.
Key words: Low molecular weight heparin; Endostatin; Modification; Cornea; Neovascularization; Rabbit
找一种效果更佳的内皮抑素修饰物。
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