【摘要】 目的：探讨17β雌二醇对高氧诱导的视网膜神经节细胞层（retinal ganglion cell layer,RGCL）细胞凋亡的保护作用并探讨其机制。方法：7天龄SD大鼠48只随机分为4组，每组l2只，分为正常对照组、单纯高氧组、雌二醇高氧组及溶媒高氧组，单纯高氧组于氧浓度750mL/L 的容器内饲养5d，再置正常空气下饲养至17天龄，雌二醇高氧组饲养方法同单纯给氧组，在大鼠7～17天龄间每日皮下注射17β雌二醇（E2，植物油稀释，配制浓度为2g/L）0.5μL/d，溶媒高氧组饲养方法同雌二醇高氧组，药物改为相同体积植物油。眼球切片并行TUNEL染色和HE染色，观察4组RGCL的细胞形态，免疫组化染色观察诱生型一氧化氮合酶（iNOS）的表达。结果：4组小鼠RGCL均见部分细胞凋亡及TUNEL染色阳性，单纯高氧组的TUNEL阳性细胞数显著多于正常组，雌二醇高氧组显著少于单纯给氧组和溶媒高氧组。视网膜免疫组织化学检测雌二醇干预组iNOS与单纯高氧组有统计学差异(P<0.05)，单纯高氧组与正常对照组有统计学差异(P<0.05)。 结论：17β雌二醇可较高氧诱导的视网膜细胞凋亡少，机制可能与NO相关。
【关键词】 17β雌二醇；凋亡；早产儿视网膜病变；诱生型一氧化氮合酶
Protective effects of 17βestradiol on retinal cell apoptosis caused by hyperxia
HaiYan Fan，Wei Jie Ni, CaiHong Shi
Department of Ophthalmology，the Third Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine， Shanghai 201900，China
Abstract
AIM：To explore the protective effects of 17βestradiol on the cell apoptosis in retinal ganglion cell layer which is induced by hyperxia.
METHODS：Fortyeitht 7dayold SD rats were divided into four groups：normal group, high oxygen group, 17βestradiol treatment group and vegetable oil treatment group. Rats of the high oxygen group were put into the environment exposed to 750mL/L oxygen for 5 days and backed to room air for another 5 days to establish the oxygen induced retinopathy(OIR)mode1.The treatment group were given 17βestradiol by injection with the dose of 0.5μL/d(2g/L）before exposed to 750mL/L oxygen. The vegetable oil treatment group is similar to 17βestradiol treatment group, but the medicine changed . Ocular crosssections were prepared for staining of HE and eyeball slicing parallel TUNEL staining. To observe the cell morphology of 4 groups RGCL. And the expression of inducible nitric oxide synthase (iNOS) of immunohis tochemical staining.
RESULTS: Part of apoptosis and positive TUNEL staining in 4 groups of mice. The number of TUNELpositive cells in pure oxygen exposure significantly more than the normal group, estradiol highoxygen group was significantly less than pure oxygen group and solvent group hyperoxia. Retinal immunohistochemistry iNOS estradiol intervention group with a simple highoxygen group has significant difference (P<0.05), hyperoxia group and normal control group has significant difference (P<0.05).
CONCLUSION: 17βestradiol have protective effects on cell apoptosi caused by hyperxia in retinal ganglion cell layer. The mechanism may relate with NO.
KEYWORDS: 17βestradiol ;apoptosis；retinopathy of prematurity； iNOS
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