深低温保存导致胎儿角膜细胞凋亡的初步研究

　　马路生，高波，高磊，毕宏广，张春虹，张秦娟，王咏梅 

　　作者单位：（264000）中国山东省烟台市，青岛大学医学院附属烟台毓璜顶医院眼科

　　作者简介：马路生，男，毕业于青岛大学医学院，医学硕士，主治医师

　　研究方向：白内障、角膜病。

　　通讯作者：马路生[email protected]

　　收稿日期：2008-04-23修回日期：2008-06-02

　　Study of the effects of deep cryopreservation on apoptosis in fetal cornea

　　Lu-Sheng Ma, Bo Gao, Lei Gao, Hong-Guang Bi, Chun-Hong Zhang, Qin-Juan Zhang, Yong-Mei Wang

　　Department of Ophthalmology, Yuhuangding Hospital of Yantai, Qingdao University, Yantai 264000, Shandong Province, China

　　Correspondence to: Lu-Sheng Ma. Department of Ophthalmology, Yuhuangding Hospital of Yantai, Qingdao University, Yantai 264000, Shandong Province, China. [email protected]

　　Received:2008-04-23Accepted:2008-06-02

　　Abstract

　　AIM: To sum the number of apoptotic cells in both normal fetal cornea and the deep cryopreservative fetal cornea and to assess the effects of deep cryopreservation to apoptosis. 

　　METHODS: The TUNEL (TdT mediated biotin dUTP nick-end labeling, TUNEL)  technique  was used to study if apoptosis was appear in both nomal fetal cornea and cryopreservative cornea in 20 fetal cornea.

　　RESULTS: The number of apoptotic cells was significantly higher after cryopreservation (7.483±2.490) than that of noncryopreservation (0.817±0.612,P<0.05).

　　CONCLUSION: Apoptosis is seldom seen in normal fetal cornea but is obvious in deep cryopreservative fetal cornea. That shows freeze-thaw trauma maybe relate to cell apoptosis.

　　KEYWORDS: apoptosis; cornea; TUNEL
Ma LS, Gao B, Gao L, et al. Study of the effects of deep cryopre-servation on apoptosis in fetal cornea. Int J Ophthalmol(Guoji Yanke  Zazhi) 2008;8(6):1137-1138

　　摘要

　　目的：研究新鲜和深低温保存后的胎儿角膜的细胞凋亡情况。

　　方法：应用TUNEL法对20例新鲜及深低温保存后胎儿角膜进行了细胞凋亡的研究。

　　结果：凋亡的角膜细胞全为细胞核着色。新鲜角膜中平均每高倍视野可见凋亡细胞0.817±0.612个，经冷冻保存后的角膜每高倍视野可见凋亡细胞平均为 7.483±2.490个，经统计学处理P<0.05,差异有显著性。

　　结论：新鲜胎儿角膜可见极少量凋亡细胞。胎儿角膜经深低温冷冻保存后，凋亡细胞数量显著增加。降温-复温损伤可能与细胞凋亡有关。

　　关键词：凋亡；角膜 ；TUNEL

　　马路生，高波，高磊，等.深低温保存导致胎儿角膜细胞凋亡的初步研究.国际眼科杂志 2008；8（6）：1137-1138