杨学义   付小兵   窦忠英

洛阳师范学院生命科学系   解放军第三O四医院全军创伤修复重点实验室 

 西北农林科技大学动物医学院   471022

Purpose:To explore the underlying mechanism of reconstructing damaged corneal surface by epidermal cells.

Method:Engineering tissue combined epidermal cells with human amniotic membrane，then，were transplanted in goats with acute full-thickness skin defect(AFTSD)and limbal stem cell deficiency(LSCD)respectively，and analyzed the p63 expression pattern of regenerated tissue from cornea and skin by confocal microscope.

Results:Results show that△αp63 positive cells clustered in the spinous and granular layers of regenerated epidermis，which were CK 1 negative，suggesting that the dedifferentiation of epidermal cells to stem cells in vivo during the process of tissue regeneration.In addition，groups of△αp63 positive，and CK12 negative cells could be seen in suprabasal layer of reconstructed cornea.

Conclusion:Those findings showed that dedifferentiation of differentiated cells to stem cells in vivo will be attributed to the process of tissue regeneration.