【摘要】 目的：解明胶壳聚糖可吸收膜支架对缓解兔眼实验性滤过泡瘢痕化的作用。方法：制备明胶壳聚糖可吸收膜，兔眼滤过手术中植入结膜下，并设立对照组。术后裂隙灯观察滤过泡情况。术后7，14d摘除眼球后常规病理HE染色，计算滤过泡腔面在结膜中形成的面积百分比，并按照独立样本t检验分析明胶壳聚糖可吸收膜对滤过泡形成及纤维化的影响。结果：术后7d实验组及对照组均可见弥散隆起的滤过泡。术后14d实验组可见滤过泡扁平，对照组滤过泡完全消失。术后7d实验组与对照组在高倍视野下滤过泡腔面积在结膜中形成的面积百分比分别为6.16%±1.02%，5.96%±0.85%，统计学分析无明显差异。术后14d实验组滤过泡腔面积在结膜中形成的面积百分比1.09%±0.13%，对照组可见大量纤维组织增生，未见滤过泡，根据独立样本t检验，t=8.52,P=0.012,实验组的滤过泡面积百分比明显高于对照组。结论：明胶壳聚糖可吸收膜的支架作用对术后滤过泡的维持及防止滤过泡瘢痕化有效。
【关键词】 吸收;膜剂;瘢痕
Alleviate the rabbits eyes of filtering bleb scarring by Chitosan gelatin absorbable stent
Kai Mu1, FangWei Ying2, Ming Li2
1Yinglihua Medicine Technology Limited Company, Shenzhen 518000, Guangdong Province,China;2Shenzhen Eye Hospital, Shenzhen 518001, Guangdong Province,China
Correspondence to:FangWei Ying.Shenzhen Eye Hospital, Shenzhen 518001, Guangdong Province,[email protected]
Received:20100608 Accepted:20100726
Abstract
AIM: To understand the effect of Chitosan gelatin absorbable stent on alleviating the rabbits eyes of filtering bleb scarring.
METHODS: Preparation of Chitosan gelatin absorbable membrane as implants were applied in rabbit eye filtration surgery under the conjunctiva ,and the control group was established. The postoperative filtering bleb condition was observed by slitlamp. Eyes were removed to stain with HE on postoperative day 7 and 14, and the percentage of the filtering bleb area in the conjunctiva was calculated,and the effect of Chitosan gelatin absorbable membrane was analyzed according to the filtering bleb formation and fibrosis by independentsamples t test .
RESULTS: In the postoperative day 7, the bulging diffuse bleb could be seen in both groups. In the postoperative day 14, flat bleb could be seen in the experimental group and no bleb but a large number of fibrous tissue in the control group. The percentage of the filtering bleb area in the conjunctiva were 6.16%±1.02%, 5.96%±0.85% in the experimental and control group, respectively in the postoperative day 7 and there was no statistical difference.However, in the postoperative day 14, The percentage of the filtering bleb area in the conjunctiva was 1.09%±0.13% in the experimental group, but no filtering bleb in the control. The percentage was higher in the experimental group than in the control group by the independentsamples t test (t=8.52 and P=0.012).
CONCLUSION: It is effective to prevent filtering bleb scarring with the Chitosan gelatin absorbable membrane.
KEYWORDS: absorption; membrane; scarring
Mu K, Ying FW, Li M. Alleviate the rabbits eyes of filtering bleb scarring by Chitosan gelatin absorbable stent. Int J Ophthalmol (Guoji
Yanke Zazhi) 2010;10(9):16751676
滤过性手术是治疗青光眼的重要方法，但术后滤过泡瘢痕化常导致手术的失败，目前的抗瘢痕化药物效果并不理想。我们利用明胶壳聚糖可吸收膜作为支架，维持术后滤过泡的形成以防止瘢痕化如下。
1材料和方法
1.1材料
健康成年新西兰白兔6只，体质量2.0～2.5kg，由广东省动物实验中心提供，手术显微镜(德国Zeiss)，明胶(上海生工)，壳聚糖(上海生工)。
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