【摘要】 目的：研究人眼眶前脂肪细胞的培养方法，并比较不同方法培养的利弊。方法：分别采用组织块法和酶消化法对人眼眶前脂肪细胞进行原代培养。结果：两种方法均能培养出大量的前脂肪细胞，并能进行传代。组织块法方法简单，需要的组织标本量少，但在原代培养时组织块容易脱离瓶壁，生长欠均匀，传代前生长较缓慢，培养的细胞成分较混杂；酶消化法获得的细胞成分较单一，增殖迅速，但步骤较复杂，需要的标本量较多。 结论：人眼眶前脂肪细胞可用组织块法和酶消化法进行原代培养，研究者可根据病例标本获得量的多寡选择不同的培养方法。
【关键词】 眼眶；前脂肪细胞；细胞培养
Contrast of two methods in culturing human orbital preadipocytes
Xiang Lei, Xue-Liang Xu
Foundation item: Fund of Hunan Provincial Scientific Technical Office, China (No. 05JJ-148)
Department of Ophthalmology, Xiangya Hospital, Central-south University, Changsha 410008, Hunan Province, China
AbstractAIM: To study the methods in culturing human orbital preadipocytes, compare advantages and disadvantages of them. METHODS: Tissue culture methods and enzyme - digesting methods were used in the primary cell culture of human orbital preadipocytes. We compared these two methods through cells isolation, cells culture, immuno phenotypic analyses and adipogenic differentiation.· RESULTS: These were two methods reliable to obtain enough preadipocytes which could proliferate and had limited passages. Tissue culture methods were simpler. But the samples were easy to detach from the wall of the culturing bottles when culturing the primary cells. The cells grew slower and might not grow regularly in primary culture. Enzyme - digesting methods could obtain simpler cells than tissue culture methods and the primary cells proliferated rapidly, while the procedure was complicated and more samples were needed.
CONCLUSION: Tissue culture methods and enzyme - digesting methods are both reliable for the primary cell culture of human preadipocytes. According to the quantity of the samples obtained, the researchers can choose different methods.
· KEYWORDS: orbital; preadipocyte; cell culture
0引言
前脂肪细胞是存在于正常人脂肪组织中的一种成体干细胞，有自我更新的能力，并能分化为成熟的脂肪细胞。近年来对其生物学特征的研究表明，前脂肪细胞在多个学科中都有较重要的意义：眼眶脂肪组织中的前脂肪细胞可能参与了甲状腺相关眼病（TAO）的发病过程；2型糖尿病的病变过程可能有前脂肪细胞的参与；也有研究报道，前脂肪细胞有可能成为组织工程化人工脂肪组织的种子。我们采用酶消化法和组织块法对正常人眼眶前脂肪细胞进行原代培养，以比较其优劣如下。
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