De-Sheng Fu1, Xue-Liang Xu2

    Foundation item: Fujian Provincial Science & Technology Fund, China (No.C0510018).

    1Department of Ophthalmology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China;2Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China

     Abstract AIM: To quantify the mRNA expression of CD40 induced by rhIFN-γ in cultured human retroocular fibroblast (RF) from normal subjects and thyroid associated ophthalmopathy (TAO) patients. ETHODS: The mRNA expression of CD40 induced by rhIFN-γ was half quantified in cultured human RF with reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The absorbency values of 100, 200, 400, 1 000kU/L rhIFN-γ was 0.483±0.095 and 0.476±0.051, 0.647±0.034 and 0.659±0.083, 0.849±0.017and 0.852±0.024, 0.891±0.082 and 0.894±0.041 compared to 0.203±0.032 and 0.213±0.020 of the absorbency values of 0kU/L rhIFN-γ respectively in RF from normal subjects and patients with TAO (P<0.01), with no significant difference at the same concentration (P >0.05). The absorbency values of 12, 24, 48, 72 hours of 400kU/L rhIFN-γ was 0.331±0.047 and 0.329±0.025, 0.668±0.067 and 0.683±0.104, 0.849±0.017 and 0.852±0.024, 0.865±0.021 and 0.876±0.014 compared to 0.203±0.032 and 0.213±0.020 of the absorbency values of 0kU/L rhIFN-γ respectively in RF from normal subjects and patients with TAO (P<0.05), with no significant difference at the same time (P>0.05).
CONCLUSION: The cultured human RF from both normal subjects and patients with TAO expresses CD40 mRNA. The rhIFN-γ could up-regulate CD40 gene mRNA expression in human RF from both normal subjects and patients with TAO in a concentration- and time-dependent manner.

    · KEYWORDS: retroocular fibroblast; thyroid associated ophthalmopathy; IFN-γ; CD40

      摘要目的：探讨rhIFN-γ作用下，正常人和TAO患者球后成纤维细胞（RF）CD40 mRNA的表达情况。方法：用RT-PCR法，对rhIFN-γ诱导正常人和TAO患者RF CD40的mRNA进行半定量分析。结果：经100，200，400，1 000kU/L rhIFN-γ作用48h后，正常人和TAO患者RF CD40的吸光度比值分别为0.483±0.095和0.476±0.051，0.647±0.034和0.659±0.083，0.849±0.017和0.852±0.024，0.891±0.082和0.894±0.041与各自对照组0.203±0.032和0.213±0.020相比，差异有统计学意义（P ≤0.01）；两者同一浓度相比差异无统计学意义（P ＞0.05）。经400kU/L rhIFN-γ作用12，24，48，72h后，正常人和TAO患者RF CD40的吸光度比值分别为0.331±0.047和0.329±0.025，0.668±0.067和0.683±0.104，0.849±0.017和0.852±0.024，0.865±0.021和0.876±0.014与各自对照组0.203±0.032和0.213±0.020相比，差异有统计学意义 （P ＜0.05），两者同一时间相比差异无统计学意义（P ＞0.05）。 结论：rhIFN-γ可上调正常人和TAO患者RF CD40 mRNA的水平，呈浓度和时间依赖。

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