CNTF和Ad-BDNF对视神经夹伤后视网膜神经节细胞存活的影响
崔志利1，康军2，惠延年1，胡丹1
基金项目：中国人民解放军兰州军区科研基金项目（No.LXH-2005008）
作者单位：1（710032）中国陕西省西安市，第四军医大学西京医院眼科；2（710054）中国陕西省西安市，解放军第451医院眼科
作者简介：崔志利，男，博士，研究方向：眼外伤。
通讯作者：胡丹，男，博士后，硕士生导师，研究方向：视神经损伤. [email protected]
收稿日期：2008-01-06修回日期：2008-05-15
Effects of CNTF and Ad-BDNF on survival of RGC after optic nerve injury
Zhi-Li Cui1, Jun Kang2, Yan-Nian Hui1, Dan Hu1
Foundation item: Science Research Project of Lanzou Military District Chinese PLA (No. LXH-2005008 )
1Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xian 710032, Shaanxi Province, China; 2 Department of Ophthalmology,No.451 Hospital of Chinese PLA, Xian 710054,Shaanxi Province, China
Correspondence to: Dan Hu. Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xian 710032, Shaanxi Province, China. [email protected]
Received: 2008-01-06Accepted: 2008-05-15
Abstract
AIM: To investigate the effect of intravitreal injection of CNTF and Ad-BDNF on survival of RGC after optic nerve injury.
METHODS: Optic nerve crush model in rats were established. CNTF and Ad-BDNF were intravitreal injected by retrograde marking the RGC with fluoro-gold (FG). The retrograde labeled RGC were counted under fluorescent microscope.
RESULTS: The number of RGC counted by retrograde labeling of FG was about 2155±265个/mm2 (n=12) after label. Number of RGC decreased quickly after crush 1-2 weeks. In CNTF group, the number of RGC was higher than that of control group (P<0.05). But the number of RGC 2-4 weeks after crush in CNTF group had no difference with that in control group. In Ad-BDNF group, the number of RGC 2-4 weeks after crush were significant difference compared with that of control group at every time point (P<0.05 ).
CONCLUSION: Intravitreal injection of CNTF can protect the RGC in the early time. But these effects can not work for more than 2 weeks. Intravitreal injection of Ad-BDNF can protect the RGC in 4 weeks.
KEYWORDS: optic nerve; crush; CNTF; Ad-BDNF; retinal ganglion cell; fluoro-gold
Cui ZL, Kang J, Hui YN, et al. Effects of CNTF and Ad-BDNF on survival of RGC after optic nerve injury. Int J Ophthalmol(Guoji Yanke Zazhi)2008;8(6):1130-1132
摘要
目的：观察大鼠视神经夹伤后玻璃体腔内注射睫状神经营养因子（CNTF）和腺病毒介导脑源性神经营养因子（Ad-BDNF）对视神经损伤后视网膜神经节细胞（RGC）存活的影响。
方法：制作大鼠视神经定量夹伤模型，玻璃体腔内注射CNTF和Ad-BDNF，经上丘荧光金（FG）逆行标记RGC，计数视网膜铺片上的RGC并行统计学分析。
结果： 正常SD大鼠视网膜上RGC密度为2155±265个/mm2(n=12)，视神经夹伤后RGC在1～2wk内下降速率最快，到3,4wk时RGC细胞数量虽仍有减少但下降速度已经明显减慢。CNTF组在视神经夹伤后1wk时视网膜RGC数显著高于对照组，但2～4wk的结果和对照组比较差异不明显。Ad-BDNF组视神经夹伤后1～4wk视网膜RGC数均显著高于对照组。
结论：CNTF治疗组玻璃体腔内一次性注射CNTF可以在损伤早期2wk内为损伤的RGC提供神经营养因子，减少RGC的早期死亡。Ad-BDNF治疗组的这种保护作用可以持续到损伤后4wk，能够为RGC提供长时间地营养支持，但这种作用比较局限，可能与单一营养因子作用有关。
关键词：视神经；夹伤；睫状神经营养因子；腺病毒介导脑源性神经营养因子；视网膜神经节细胞；荧光金
崔志利,康军,惠延年,等. CNTF和Ad-BDNF对视神经夹伤后视网膜神经节细胞存活的影响.国际眼科杂志 2008;8(6):1130-1132 |
