姜彩辉 张卯年 Henry Klassen Michael Young

　　解放军总医院眼科 Schepens Eye Research Institute Harvard Medical School Department of Ophthalmology School of Medicine University of California Irvine Orange CA 92868 100853

　　PURPOSE.To investigate integration，differentiation and synaptic connectivity of RPCs transplanted into the subretinal space of mice with laser-induced damage。

　　METHODS.RPCs were harvested from the neural retinas of postnatal day 1 mice.Photocoagulation was performed and RPC suspension were injected into the subretinal space。

　　RESULTS.Three weeks after transplantation，1158±320 cells per eye had migrated into the ONL.Most of these cells resided in the ONL and some had developed morphological features typical of mature photoreceptors.Most of the GFP+cells co-labeled with rhodopsin and recoverin.Some developed outer segments expressing rhodopsin，peripherin-2，and rom-1.A subset of these integrated cells expressed bassoon and made synaptic contact with rod bipolar cells.Retinal photocoagulation resulted in significantly increased expression of MMP-2，MMP-9，and CD44s，and decreased expression of neurocan。

　　CONCLUSONS.Transplanted RPCs migrate and integrate into the laser-injured ONL where they differentiate into photoreceptors with mature morphological features，express synaptic protein，and form synaptic connections with retinal bipolar cells.