2 结果
2.1 巨噬细胞吞噬消化功能
角膜碱烧伤后,腹腔巨噬细胞体积增大明显,吞噬力和消化力明显增强;TGF-β1治疗组,巨噬细胞吞噬力在5周内明显低于对照组(表1,表2)。
表1 TGF-β1应用组与对照组巨噬细胞吞噬率的比较(%, ±s)
Tab.1 A comparison between TGF-β1 injected and
control group in phagocytosing rate(%, ±s)
Time(week) |
Control group |
TGF-β1 group |
P |
1 |
22.02±1.48 |
15.72±0.08 |
<0.05 |
3 |
23.54±1.70 |
12.97±2.46 |
<0.05 |
5 |
19.36±0.80 |
13.84±1.29 |
<0.05 |
7 |
20.37±1.10 |
19.60±0.86 |
>0.05 |
表2 TGF-β1应用组与对照组巨噬细胞吞噬指数的比较( ±s)
Tab.2 A comparison between TGF-β1 injected and control
group in phagocytosing index ( ±s)
Time(week) |
Controlgroup |
TGF-β1group |
P |
1 |
0.29±0.01 |
0.23±0.03 |
>0.05 |
3 |
0.30±0.02 |
0.17±0.03 |
<0.05 |
5 |
0.22±0.02 |
0.16±0.02 |
<0.05 |
7 |
0.33±0.03 |
0.26±0.02 |
>0.05 |
2.2 淋巴细胞转化功能
2.2.1 PHA刺激:TGF-β1治疗组,△cpm,RPI,SI在1,3周时明显较对照组降低(表3,表4)。
表3 PHA刺激时TGF-β1应用组与对照组△cpm的比较( ±s)
Tab.3 A comparison between TGF-β1 injected and
control group in lymphocyte blastogenesis(△cpm)
induced by PHA( ±s) |
Time(week) |
Controlgroup |
TGF-β1group |
P |
1 |
16 950.88±5 776.97 |
3 606.67±2 025.298 978.01±3 054.40 |
<0.05 |
3 |
13 089.78±4 832.27 |
|
<0.05 |
5 |
15 993.22±6 025.74 |
9 717.41±7 047.55 |
>0.05 |
7 |
18 164.70±6 335.76 |
14 445.04±8 932.61 |
>0.05 |
表4 PHA刺激时TGF-β1应用组与对照组RPI,SI的比较( ±s)
Tab.4 A comparison between TGF-β1 injected and
control group in lymphocyte blastogenesis(RPI,SI)
induced by PHA( ±s)
Time
(week) |
RPI |
SI |
Control |
TGF-β1 |
P |
Control |
TGF-β1 |
P |
1 |
1.73±0.59 |
0.37±0.21 |
<0.05 |
2.08±0.5 |
11.06±0.26 |
<0.05 |
3 |
1.41±0.47 |
0.92±0.31 |
<0.05 |
1.45±0.2 |
41.19±0.21 |
<0.05 |
5 |
1.64±0.62 |
1.05±0.66 |
>0.05 |
1.32±0.2 |
51.21±0.25 |
>0.05 |
7 |
1.86±0.65 |
1.48±0.91 |
>0.05 |
1.62±0.21 |
1.50±0.31 |
>0.05 |
2.2.2 烧伤角膜蛋白刺激:TGF-β1治疗组与对照组相比,差别无显著性(P>0.05)。
2.3 角膜组织切片光镜检查
TGF-β1治疗组,烧伤角膜增厚明显,上皮细胞异常增生,基质纤维母细胞及胶原纤维板层显著增多,新生血管生长旺盛;对照组烧伤角膜上述变化不显著。两组均未见明显的炎症细胞浸润。
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