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2 结果
Fas抗原在正常全厚视网膜内均有表达，在神经纤维层较强。三种神经元细胞亦有表达，视网膜色素上皮(retinal pigment epithelium,RPE)细胞表达较弱(图1)。FasL在RPE细胞、视杆和视锥细胞呈弱阳性。外丛状层表达明显，向内颗粒层逐渐减弱。在视网膜神经纤维层可见梭形细胞表达FasL(图2)。TUNEL检测在全层视网膜未见凋亡细胞。
PVR C级5例，PVR D级6例，对玻璃体切割液细胞离心涂片的免疫组织化学染色均有Fas抗原强阳性表达的细胞(图3)。阳性细胞占所有细胞的比例为：(70.35±27.60)%(27.40%～96.52%)，PVRC级和D级间阳性细胞百分率无差别(P＞0.05)。11例的细胞标本均见FasL强阳性表达的细胞(图4)，阳性细胞百分率为(51.58±26.47)%(17.65%～83.80%)。凋亡细胞(图5)所占的比例为(20.53±12.58)%(2.00%～36.36%)。Fas表达的细胞阳性率与凋亡细胞率的直线相关系数r值为0.99(P＜0.001)；FasL与凋亡细胞的r值为0.64(0.001＜P＜0.005)；Fas与FasL的r值为0.77(P＜0.05)。CK阳性细胞率为：(82.97±4.76)%(76.00%～90.00%)(表1)。
  
 
图1 正常人眼视网膜Fas表达。视网膜呈Fas阳性，RPE细胞(箭)反应较弱 SP×100 图2 正常人眼视网膜FasL表达。RPE细胞(箭)弱表达，神经纤维层可见一梭形阳性细胞(箭头) SP×100 图3 PVR D1级玻璃体切割液细胞离心涂片Fas表达。Fas阳性细胞(箭)含色素颗粒，阴性细胞(箭头) SP×132 图4 PVR C2级玻璃体切割液细胞离心涂片FasL表达。FasL阴性细胞(箭头)呈成纤维细胞样，阳性细胞(箭) SP×132 图5 PVR D2级玻璃体切割液细胞离心涂片凋亡细胞。凋亡细胞含深蓝色细胞核(箭)，阴性细胞(箭头) TUNEL×132 Fig.1 Fas antigen expression in a normal control retina．Fas-positive retinal and weaker immunoreactivity in RPE cell(arrow) SP×100 Fig.2 FasL expres-
sion in a normal control retina.RPE cell(arrow)is also weaker in immunoreactivity.A spindle FasL-positivecell(arrowhead)in optic fiber layer SP×100 Fig.3 Fas antigen expression in a vitrectomy cytocentrifuge slide of a patient with PVRD1.Fas-positive cells(arrow)contain some pigments.Fas-negative cell(arrowhead) SP×132 Fig.4 FasL expression in a vitrectomy cytocentrifuge slide of a patient with PVRC2.Fas-negative cells(arrowhead)are fibroblast-like.Positive cell(arrow) SP×132 Fig.5 Apoptosis in a vitrectomy cytocentrifuge slide of a patient with PVR D2.A apoptotic cell with a darkly blue nucleus(arrow)and some negative cells(arrowhead) TUNEL×132
表1 11例PVR玻璃体切割液离心标本Fas，FasL，CK及TUNEL阳性细胞表达
Tab． 1 Positive expressions of Fas,FasL,CK，and TUNEL in vitreous specimens
| Case |
Sex |
Age |
Course
(mon) |
PVR |
Positive cells(%) |
| Fas |
FasL |
CK |
TUNEL |
| 1 |
M |
27 |
5.0 |
D1 |
90.20 |
82.75 |
80.01 |
27.37 |
| 2 |
F |
13 |
1.0 |
C2 |
96.52 |
83.87 |
79.20 |
36.36 |
| 3 |
F |
56 |
3.0 |
D1 |
86.40 |
77.30 |
82.50 |
30.19 |
| 4 |
F |
50 |
4.0 |
D2 |
76.40 |
60.30 |
87.10 |
11.11 |
| 5 |
M |
57 |
0.5 |
C3 |
94.25 |
80.67 |
76.02 |
32.36 |
| 6 |
M |
17 |
1.0 |
D1 |
85.90 |
26.40 |
90.00 |
29.17 |
| 7 |
M |
25 |
3.0 |
D1 |
87.10 |
38.50 |
77.20 |
31.20 |
| 8 |
F |
61 |
8.0 |
C3 |
29.10 |
27.40 |
84.80 |
6.00 |
| 9 |
F |
60 |
0.8 |
C3 |
27.40 |
28.33 |
80.90 |
2.00 |
| 10 |
F |
40 |
0.5 |
C3 |
30．30 |
17.65 |
89.30 |
10.00 |
| 11 |
F |
20 |
12.0 |
D1 |
70.25 |
50.20 |
85．70 |
10.07 |
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