丁阳   闫东升   周仲楼   安建宏   周翔天   瞿佳   吕帆

温州医学院附属眼视光医院   325027

PURPOSE：Many studies have indicated that sclera is a possible site of action for the currently used muscarinic acetylcholine receptor(mAChR)antagonists in prevention of myopia.The aim of this study，therefore，was to determine effect of muscarinic agents in human scleral fibroblasts(HSF)proliferation and regulation of MMP-2 and collagen。

METHOD：Primary cells were harvested by trypsinization from human donors.Cultured HSF were used at passage 4-7 and were treated with various concentrations of atropine(nonselective mAChR antagonist).The proliferation of HSF treated with atropine was measured by MTT.The expression of MMP-2 and collagenⅠwas examined by real-time PCR.

RESULT：MTT results indicated that atropine inhibited proliferation of HSF.The expression of MMP-2 in cultured HSF was significantly increased by addition of 0.1M and 1M atropine for 24 and 48 hours，and the content of collagenⅠwas decreased after 48 hours.

CONCLUSIONS：Our results obtained from in vitro assays in the cultured cells demonstrate that mscarinic agents are able to mediate sclera growth and remodeling.It is speculated that maybe the site of action of mAChR antagonist is not in sclera directly.