Integrin-linked kinase(ILK)regulated VEGF secretion in vitro and the expression of ILK in ischemia-induced retinopathy

　　于文贞 黄旅珍 黎晓新

　　北京大学人民医院 100044

　　Purpose:This study was performed to investigate the function of Integrin-linked kinase(ILK)and whether the ILK can be expressed in the mouse model of ROP.

　　Method:We examined the response of RF/6A cells to hypoxia and employed the small interfering RNA(siRNA)to knock down gene expression of ILK in RF/6A cells in vitro.In vivo studies，ROP hyperoxia/hypoxia model was used.Normal mice were exposed to roomair.The ILK，VEGF and HIF of RF/6A cells were studied by real-time RT-PCR，western blot and ELISA after treatment with or without siRNA specific for ILK under hypoxia.The relative levels of ILK in the eyes from normal mice and ROP mice were determined by real-time RT-PCR，Western blot and immunohistochemistry.

　　Results:Both the mRNA and protein levels of ILK in the RF/6A cells increased in response to hypoxia，followed by increasing expression of HIF-1a and VEGF.The ILK siRNA decreased the mRNA and protein levels of ILK，HIF-1a and VEGF in the RF/6A cells.In vivo，the mRNA and protein expression levels of ILK were increased during the development of neovascularization.

　　Conclusion:ILK may be involved in angiogenesis by controlling the expression of HIF-1a and VEGF in vivo and in vitro.