Transfection of NgR1 shRNA improves axonal regeneration of optic nerve after crush

　　苏颖 滕岩 王峰 刘平 崔浩

　　哈尔滨医科大学附属第一医院 150001

　　Purpose:Axonal regeneration of retinal ganglion cells(RGCs)was investigated in vivo and vitro after transfection with Nogo receptor(NgR)shRNA.

　　Method: For experiments related with shRNA-mediated knockdown of NgR gene，retinal ganglion cells were transfected in vivo and vitro with NgR shRNA.The left and right ON was crushed.After withdrawing 2μl of fluid from each of both eyes，1μl of shRNA(experimental group)，negative control(negative control group)or PBS(blank control group)was carefully injected into the vitreous body.NgR shRNA and negative control were added into medium for culturing RGCs.After transfection，all the RGCs were cultured 7 days in vitro.

　　Results:There is little expression of NgR after transfection of NgR shRNA.The difference in axonal length between group A and group B was significant at either the third day or the seventh day.

　　Conclusion:NgR genes play an inhibitive role in the axonal regeneration of RGCs，while transfection of NgR shRNA is an effective way to eliminate this inhibition and accelerate axonal regeneration.