于旭辉
哈尔滨医科大学附属第一临床医院 030001
Purpose:To screen the potent clinical biomarkers in DR,total transcriptome of diabetic retina of rats were studied and optimal individual candidate was furtherly evaluated.
Method:SAGE was used as the initial screen to find optimal biomarkers in streptozotocin-induced diabetic rats.Glutamine synthetase(GS)furtherly measured at various time points after diabetes induction.Northern blot,RT-PCR and colorimetric enzyme activity assays were synchronously used to compare the differences between diabetic retinopathy rats and healthy controls.
Results:AGE observed a 45.6%reduction in transcript levels of GS in diabetic rats compared with the normal.RT-PCR and enzyme activity assays revealed significant differences in GS mRNA expression and activity.Northern blot analysis indicated a linear correlation between the reduction in GS expression and the time course of diabetic retinopathy(r=0.802,p<0.0001),which was validated by real-time RT-PCR(r=0.731,p<0.001).
Conclusions:Our results implicate GS as a potential biomarker for evaluating the severity of diabetic retinopathy over the time course of diabetes progression.
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