2 结果
2.1 晶状体混浊情况:非半乳糖腹腔注射25只大鼠晶状体始终保持透明,腹腔注射半乳糖30只大鼠,30天后均有不同程度的晶状体混浊,但维拉帕米治疗的C组大鼠晶状体混浊速度较未予治疗的D组慢,程度明显减轻,且均未达到IV期,而D组几乎所有动物30天后晶状体混浊超过IV期(表1)。
2.2 血清中Na+,K+,Ca2+浓度变化:30天后,各组动物血清中Na+,K+浓度无明显差异(表2);未经治疗的D组血清中Ca2+浓度明显高于对照组(A,B组)(P<0.05),而经治疗的C组血清Ca2+浓度亦高于A,B组,但无统计学差异。
表1 半乳糖腹腔注射C,D组大鼠晶状体变化比较
Tab.1 The comparison of lens opacity between
group C and D with galactose injection
|
n |
7d |
14d |
21d |
30d |
≤Ⅰ |
>Ⅰ |
≤Ⅱ |
>Ⅱ |
<Ⅲ |
Ⅲ |
>Ⅲ |
≤Ⅳ |
>Ⅳ |
Group C |
15 |
15 |
- |
11 |
4 |
3 |
12 |
- |
- |
- |
Group D |
15 |
2 |
13 |
1 |
14 |
- |
- |
15 |
2 |
13 |
表2 各组动物血清中Na+,K+,Ca2+浓度比较(±s)
Tab.2 The comparison for Na+,K+,Ca2+
concentrations of sera in four groups(±s)
|
n |
Na+(μg/ml) |
K+(μg/ml) |
Ca2+(μg/ml) |
Group A |
10 |
978.15±60.75 |
394.08±198.95 |
91.02±24.26 |
Group B |
15 |
960.07±220.65 |
315.14±126.86 |
89.87±23.34 |
Group C |
15 |
867.97±173.53 |
369.96±449.14 |
118.17±24.14 |
Group D |
15 |
931.68±32.053 |
64.29±65.051 |
35.81±15.52b |
bP<0.05 vs group A and B2.3 晶状体中水含量变化:形成白内障的C,D组晶状体水含量明显高于对照组(A,B组),而C组与D组间无显著性差异,但C组水含量有减轻的趋势(表3)。
表3 各组动物晶状体湿重、干重及水含量比较(±s)
Tab.3 The comparison for wet weight,dry weight and water
content of lens in four groups(±s)
|
n |
Wet we ight
(mg) |
Dry weight
(mg) |
Water content
(mg/mg dry weight) |
Group A |
10 |
31.7±3.0 |
10.1±0.5 |
2.14±0.39 |
Group B |
15 |
34.7±1.6 |
10.5±0.8 |
2.32±0.23 |
Group C |
15 |
32.9±2.2 |
8.3±0.7 |
3.00±0.29 |
Group D |
15 |
31.7±6.4 |
7.1±1.0c |
3.57±1.40cd |
cP<0.01 vs group A and B,dP>0.05 vs group C
2.4 晶状体内Na+,K+,Ca2+含量改变:形成白内障的C,D组晶状体中Ca2+含量明显高于非白内障组(A,B组),经维拉帕米治疗的C组晶状体中Ca2+含量明显低于D组。C,D组晶状体Na+含量较A,B组有不同程度的升高,而K+含量有不同程度的降低,且以D组较为明显(表4)。
表4 各组动物晶状体中Na+,K+,Ca2+及Na+/K+比较(±s)
Tab.4 The comparison for Na+,K+,Ca2+ contents and
Na+/K+ ratios of lens in four groups(±s)
|
n |
Ca2+(μg/10 mg
dry weight) |
Na+(μg/mg
dry weight) |
K+(μg/mg
dry weight) |
Na+/K+ |
Group A |
10 |
7.93±3.57 |
5.88±1.41 |
12.83±1.42 |
0.45±0.06 |
Group B |
15 |
7.21±1.33 |
6.63±0.68 |
13.72±2.02 |
0.48±0.10 |
Group C |
14 |
9.94±2.57 |
7.34±0.32 |
4.01±0.75 |
1.89±0.39b |
Group D |
15 |
19.34±5.66bc |
11.60±4.22b |
2.896±0.65bc |
3.98±1.34bc |
b P<0.05 vs group A and B,cP<0.01 vs group C
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