|
△P>0.05,bP<0.01
Table 3 Quantitative results ong>ofong> HLECs (smear) Immunohistochemical morphometry（略）
△P>0.05,bP<0.01
Measurement Indexes Cell area (AREA), mean optical density (M.O.D.) and integral optical density (I.O.D.).
RESULTS
Qualitative Analysis The results ong>ofong> immunohistochemistry (ABC method) showed that bFGF ong>receptorong> ong>proteinong> existed in the epithelial cells from the paraffin sections, the lens capsule slides as well as the cell smears (Figure 1, 2 and 3).
Quantitative Analysis The results ong>ofong> image analysis showed that there was significant difference in bFGF ong>receptorong> ong>proteinong> (M.O.D. and I.O.D.) between the paraffin sections, the lens capsule slides as well as the cell smears and their corresponding controls (Table 1, 2 and 3).
DISCUSSION
bFGF ong>receptorong> consists ong>ofong> an extracellular ligand binding region containing three immunoglobulinlike domains, a helical transmembrane domain and an intracellular split tyrosine kinase domain. Four FGF ong>receptorong> genes produce 48 ong>receptorong> isomers by natural alternative splice [2]. The expression ong>ofong> FGFR1 in lens epithelial cells decreases with the increase ong>ofong> age, increases after the application ong>ofong> FGF and plays an important role in cell ong>growthong> and fibrosis [3]. However, experimental study shows that the elimination ong>ofong> FGFR1 gene does not affect the normal development ong>ofong> lens [4]. FGFR2 does not affect lens epithelial cell proliferation but affect the fibrosis ong>ofong> cells only [5]. It is proposed that FGFR3 plays an important role in the fibrosis ong>ofong> lens epithelial cells [6] and FGFR4 in lens epithelial cells is FGF19 ong>receptorong> [7].
FGF ong>receptorong> exists not only in lens epithelial cells but also in baby rat kidney cells, endothelial cells, ong>fibroblastong>,cardioblast,pheochromocytoma cells and other tumor cell membranes. These ong>receptorong> ong>proteinong>s are singlechain polypeptides with molecular weights varying in the range ong>ofong> 110150kPa in different cells. FGF ong>receptorong> plays a role through MAPK/ERK1/2 signal system [8] and the genovariation ong>ofong> heparan sulfate biosynthetic gene interferes with the combination ong>ofong> FGF with its ong>receptorong> to adjust FGF effect downward [9].
The results ong>ofong> the qualitative and quantitative immunohistochemistry ong>ofong> paraffin sections, cell smears and capsule slides show that bFGF ong>receptorong> ong>proteinong> exists on HLECs, which is the basis for bFGF and its ong>receptorong> to have biological effect on HLECs. Since ong>growthong> ong>factorong> and ong>receptorong> have their final effects through their ong>proteinong>s, the level ong>ofong> ong>proteinong>s is a key ong>factorong> in determining their biological effect. bFGF in eyes might combine with the ong>receptorong> ong>proteinong>s on residual lens epithelial cell membrane during cataract removal, and therefore improve the proliferation ong>ofong> lens epithelial cells, which is the main cause ong>ofong> the formation ong>ofong> after cataract.
Figure 1 Positivereaction bFGF ong>receptorong> ong>proteinong> in a lens epithelial cell ong>ofong> a paraffin section from a 5month embryo (ABC×400)（略）
Figure 2 Positivereaction bFGF ong>receptorong> ong>proteinong> in a lens epithelial cell ong>ofong> an anterior capsule slide from a 65yearold senile cataract patient (ABC×400)（略）
Figure 3 Positivereaction bFGF ong>receptorong> ong>proteinong> in a lens epithelial cell ong>ofong> a cell smear slide from a 30yearold adult (ABC×400)（略）
【参考文献】
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2 Duchesne L, Tissot B, Rudd TR, Dell A, Fernig DG. Nglycosylation ong>ofong> ong>fibroblastong> ong>growthong> ong>factorong> ong>receptorong> 1 regulates ligand and heparan sulfate coong>receptorong> binding. J Biol Chem 2006;281(37):2717827189
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4 Zhao H, Yang Y, Partanen J, Ciruna BG, Rossant J, Robinson ML. Fibroblast ong>growthong> ong>factorong> ong>receptorong> 1 (Fgfr1) is not essential for lens fiber differentiation in mice. Mol Vis 2006;12:1525
5 Garcia CM, Yu K, Zhao H, AsheryPadan R, Ornitz DM, Robinson ML, Beebe DC. Signaling through FGF ong>receptorong>2 is required for lens cell survival and for withdrawal from the cell cycle during lens fiber cell differentiation. Dev Dyn 2005;233(2):516527
6 Govindarajan V, Overbeek PA. Secreted FGFR3, but not FGFR1, inhibits lens fiber differentiation. Development 2001;128(9):16171627
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8 Iyengar L, Wang Q, Rasko JE, McAvoy JW, Lovicu FJ. Duration ong>ofong> ERK1/2 phosphorylation induced by FGF or ocular media determines lens cell fate. Differentiation 2007;75(7):662668
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人晶状体上皮细胞成纤维细胞生长因子受体蛋白的检测
刘玉福，刘宏伟1, 上一页 [1] [2] |
