【摘要】目的：通过分析细胞因子的性质，旨在探讨前葡萄膜炎可能的发病机制。方法：取20例HLAB27相关前葡萄膜炎患者、20例小柳原田患者外周血和正常人作为对照组，通过酶联免疫反应（ELISA）检测患者外周血IFNγ，IL4和IL10的含量。结果：两组患者外周血IFNγ的含量较正常人显著增高（P<0.05），且小柳原田患者外周血中IFNγ含量显著高于HLAB27相关前葡萄膜炎患者外周血中IFNγ含量(P<0.05)。结论：前葡萄膜炎发病机制可能和对自身抗原诱发的Th1型免疫反应过强有关。
【关键词】 HLAB27相关前葡萄膜炎；小柳原田病；细胞因子
Study on the cytokines in the peripheral blood of the patients with anterior uveitis
YaLin Ren, Chong Wang, Yu Lin
Foundation item: Fund of Science and Technology of Zhongshan City, Guangdong Province, China (No. 20071C015)
1Sun Yatsen University, Guangzhou 521600, Guangdong Province, China;2Department of Ophthalmology, Zhongshan Hospital Affiliated to Sun Yatsen University, Zhongshan 528403, Guangdong Province, China;3Department of Ophthalmology, Healthcare Hospital for Women and Children of Nanshan District, Shenzhen 518000, Guangdong Province, China
Correspondence to: YaLin Ren. Department of Ophthalmology, Zhongshan Hospital Affiliated to Sun Yatsen University, Zhongshan 528403, Guangdong Province, China. [email protected]
AbstractAIM: To study the possible mechanism of anterior uveitis by evaluating the quality of cytokines in peripheral blood.METHODS: Peripheral blood was drawn from 20 patients with HLAB27 positive uveitis, 20 patients with VKH syndrome and 20 normal people. The amount of IFNγ, IL4 and IL10 were evaluated respectively with ELISA.RESULTS: The amount of IFNγ in the patients was much higher than that in the normal people (P<0.05), and the amount of IFNγ in the patients with VKH syndrome was much higher than that in the patients with HLAB27 positive uveitis (P<0.05). The content of IL4 was less than 2.6ng/L in three groups, whereas that of IL10 was less than 2.8ng/L in three groups. There was no significant difference among three groups.CONCLUSION: The anterior uveitis might be related to the enhanced Th1 immune response.
KEYWORDS: HLAB27 anterior uveitis; VKH syndrome;
cytokine
引言
前葡萄膜炎是我国比较常见的一种前葡萄膜炎，病因和类型多种多样，如伴全身疾病的前葡萄膜膜炎、免疫应答所致的特发性前葡萄膜炎、与HLAB27 相关的前葡萄膜炎以及眼肿瘤所致的伪装综合征等。过去人们通过建立自身反应性葡萄膜炎模型进行研究发现，葡萄膜炎的发生可能和Th1 型细胞因子有关，但是在人类，葡萄膜炎类型多种多样，不同类型的葡萄膜炎患者其发病机制是否和动物类似还不清楚。本研究对200703/200707前来中山医院眼科就诊的HLAB27相关前葡萄膜炎患者和小柳原田病（VKH）患者的外周血进行研究，通过分析其细胞因子的性质，旨在探讨前葡萄膜炎可能的发病机制。
1 材料和方法
1.1材料
200703/200707前来中山医院眼科就诊活动期HLAB27相关前葡萄膜炎患者20例和小柳原田患者20例分别分为1，2组。其中HLAB27相关前葡萄膜炎患者全部为男性，年龄28～35（平均31）岁；小柳原田患者中男10例，女10例，年龄22～32（平均30）岁。HLAB27相关前葡萄膜炎患者，HLAB27均为阳性，且排除全身性疾病和眼部肿瘤；小柳原田病的诊断根据2001年国际葡萄膜炎协会指定的标准进行诊断，7例患者均为不完全型小柳原田病前房受累期。同时设立20名正常人作为对照组，年龄19～35（平均31）岁。人IFNγ、IL4和IL10的ELISA试剂盒购自BDBioscience公司（U.S.A.）；RPMI1640培养液和Hanks缓冲液购自GIBCO公司（U.S.A.）。
1.2方法
1.2.1外周血单核细胞的分离
抽取以上患者和正常人外周血肝素抗凝，加等量Hanks缓冲液稀释，混匀后重层于葡聚糖泛影葡胺，900g，离心机（KDC2046离心机）24℃离心35min。离心后，吸取单核细胞（PBMCs）层，用Hanks缓冲液洗涤2次后，加入RPMI1640完全培养基悬浮细胞。
1.2.2酶联免疫检测
按照试剂盒说明书操作，每个样品做3个复孔，使用BIOTEK ELX800酶标仪（德国）进行检测分析。酶联免疫（ELISA）检测IFNγ,IL4和IL10的最低下限分别为7.1，2.6，2.8ng/L。
统计学处理：采用SPSS 11.0 统计软件包对数据进行统计处理，采用t检验对各组数据进行统计学分析，检验水准取α=0.05。
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